10/07/2019 PolyFect Transfection Reagent - QIAGEN Online Shop
https://www.qiagen.com/nl/products/discovery-and-translational-research/functional-and-cell-analysis/transfection/polyfect-transfection-reagent/#productdetails 1/2
Home−Products−Discovery&TranslationalResearch−Functional&CellAnalysis−Transfection−PolyFectTransfectionReagent
PolyFectTransfectionReagent
ForfastandeasyDNAtransfectionofstandardcelllines
Optimizedtransfectionusingcell-specificprotocols
Fastprocedureandeasyhandling
Highcellviability,lowcytotoxicity
PolyFectTransfectionReagentisbasedonactivateddendrimertechnologyoptimizedforthe
transfectionofDNAintoCOS-7,NIH/3T3,HeLa,293,andCHOcells.
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ProductDetails
Specifications ProductResources
PolyFectReagentwithHeLacells.
Expressionof[A]beta-galactosidaseand[B]greenfluorescentprotein(GFP)inHeLacellsis
shown.Cellswerecotransfectedin6-wellplateswithbeta-galactosidase-andGFP-reporter
plasmidsusingPolyFectTransfectionReagentandtheHeLacellprotocol.Expressionwas
visualizedbyX-galstainingorfluorescencemicroscopy2dayspost-transfection.
Performance
PolyFectReagentconsistentlydelivershightransfectionefficiencies(seefigures"HightransfectionefficienciesinCHOand293cells"and
"PolyFectReagentwithHeLacells").Incontrasttomanyliposomalreagents,PolyFectReagentenablestransfectioninthepresenceofserum
withoutloweringoftransfectionefficiency.
Principle
PolyFectTransfectionReagentisasolutionofspecificallydesignedactivated-dendrimers.Thereagentconsistsofdendrimermoleculesofadefined
sphericalarchitecturewithbranchesradiatingfromacentralcore(seefigure"Activateddendrimer").Thebranchesterminateatchargedamino
groups,whichcaninteractwithnegativelychargedphosphategroupsofnucleicacids.PolyFectReagentassemblesDNAintocompactstructures(see
figure"PolyFect–DNAinteraction"),thatbindtothecellsurfaceandaretakenintothecellbynonspecificendocytosis.ThereagentbuffersthepHof
theendosome,leadingtopHinhibititionofendosomalnucleases,whichensuresstabilityofPolyFect–DNAcomplexes.
Procedure
PolyFectTransfectionReagentisprovidedasaready-to-usesolution.Thetransfectionprocedure(seeflowchart"PolyFecttransfection
procedure")isfastandsimple—justaddPolyFectReagenttotheDNAsolution,mix,incubatefor5–10minutes,addgrowthmedium(whichcan
containserumandantibiotics),andpipetthePolyFect–DNAcomplexesontothecells.Thecellsarethenincubatedforgeneexpression.Nopost-
transfectionremovalofcomplexesormediumchange/additionisnecessary,makingtheprocedurefastandeasy.
High-throughputtransfection
TransfectionusingPolyFectReagentrequiresminimalhandlingandremovaloftransfectioncomplexesisnotrequired,makingthisreagenthighly
suitableforhigh-throughputscreening.PolyFectReagentprovidesoutstandingtransfectionefficiencies,excellentreproducibility,andminimal
cytotoxicityinhigh-throughputtransfection,andisavailableinbulkquantities.ForoptimizedprotocolsfortransfectionofCOS-7,NIH/3T3,HeLa,HeLa-
S3,293,andCHOcellsin96-wellplates,pleasecontactQIAGENTechnicalServices.
Applications
PolyFectTransfectionReagentishighlysuitedforefficienttransfectionofstandardcelllinesin:
10/07/2019 PolyFect Transfection Reagent - QIAGEN Online Shop
https://www.qiagen.com/nl/products/discovery-and-translational-research/functional-and-cell-analysis/transfection/polyfect-transfection-reagent/#productdetails 2/2
Studiesongeneexpressionandfunction
Drugdiscoveryanddevelopmentstudies
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