8
Exclude Assay Wells (Outliers) or Groups from Report Generator Analysis:
Individual assay wells or entire groups/conditions can be excluded
from parameter calculations in the Report Generator. Before exporting
data from Wave 2.3, click the assay well(s) on the plate map or
double-click the group name(s) on the Group List to exclude those
wells or groups from data export. The Project Information tab displays
the plate map layout and any assay wells or groups that have been
excluded from the Excel export (Figure 7).
Frequently Asked Questions
What rate measurements are used to calculate the parameters in this Report Generator?
Parameter equations are described in Table 1
of this User Guide.
How do I remove outlier wells in the Report Generator?
Outlier assay wells must be turned OFF or reassigned in Wave 2.3 prior to Excel export. See section called
Exclude
Outliers/Groups from Analysis or the Wave User Guide for more information.
Can I use the Excel file exported from the XFp, XF96, or XF24 Analyzer?
It is recommended to export assay result data directly to the XF Report Generator from Wave 2.3 (Desktop or Controller),
however data can also be manually imported as an Excel file (*.xlsx). Excel files exported from earlier versions of Wave (Desktop
or Controller) and XF96/XF24 software are not compatible. If the Excel file has been exported from Wave but cannot be imported
to the Report Generator, please contact Seahorse Technical Support
.
If you receive an error message about Instrument Protocol (XFe96; XFe24; XF96; XF24 only)
Errors upon data import into the Report Generator are likely caused by a custom cycle in the Instrument Protocol. A Custom
Cycle refers to an additional ‘Mix’ or ‘Wait’ command step in the Instrument Protocol an assay. Custom Cycles are not part of
the standardized assay template for the Seahorse XF Cell Mito Stress Test and not supported in Report Generator analysis.
Please contact Seahorse Technical Support
if you have any additional questions regarding Custom Cycles.
Can I use baseline rate data (%) to calculate assay parameters?
Normalized (or non-normalized) OCR data is used for parameter calculations, parameter calculations using baseline rate data
(%) is not supported at this time.
Where is my ECAR data?
ECAR (Extracellular Acidification Rate) data is displayed on the Measures Sheet tab in the Seahorse XF Cell Mito Stress Test
Report Generator.
How do I analyze multiple result files in a Report Generator?
The Seahorse Multi-File XF Cell Mito Stress Test Report Generator enables the import and analysis of multiple XF Cell Mito
Stress Test assay result files. Find out more and download the Multi-File XF Report Generator here
.
Figure 7 | XFp Plate Map on the
Project Information
tab. Assay well C
has been turned off in Wave 2.3 prior
to export, therefore the
Control group
parameter calculations are based on
assay wells B and D only.